Figure 1.

NF-κB activity. Shown are the results of a DNA binding assay of the NF-κB p65 transcription factor, using two biological replicates and four readouts for each sample. NF-κB activity, a key mediator of inflammation, is elevated in the nuclear fractions of fibroblasts from the group of older donors compared to young donors (p < 0.005). The DNA binding activity corresponds to a "low-grade" inflammatory state compared to the TNFα treated positive control.