Table 1

Aged B cells are able to form blast cells after 48 hours.


Media
 LPS
 Anti-CD40


% Blast Formation
 Fold-increase
 % Blast Formation
 Fold-increase
 % Blast Formation
 Fold-increase
Experiment 1
 Young
 7
 __
 52
 8
 25
 4

Aged
 10
 __
 50
 5
 24
 3
Experiment 2
 Young
 10
 __
 71
 7
 32
 3

Aged
 20
 __
 68
 3
 38
 2

SPC from young and aged mice were harvested and cultured for 48 hours in media only, LPS, or anti-CD40 mab with murine IL-4. Blast cells were gated as in figure 5 and percent blast cells were calculated. Percent of blasts from stimulating conditions were divided by percent of blast cells in media only to produce a fold-increase. Data is representative of 3 separate experiments.

Blaeser et al. Immunity & Ageing 2008 5:15   doi:10.1186/1742-4933-5-15