Figure 2.

Splenocytes from aged mice are able to upregulate CD86 effectively upon stimulation. SPC from young, middle-aged, and aged QM mice were harvested and cultured for 24 and 48 hours. Splenocytes were stained for expression of cell surface markers. Lymphocytes were gated on FSC/SSC and B cells on B220⁺. Representative of 4 separate experiments.