Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels

doi:10.1186/1742-4933-3-7

Immunity & Ageing

Volume 3

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Izzo V
Costa MA
Di Fiore R
Duro G
Bellavia D
Cascone E
Colombo P
Gioviale MC
Barbieri R

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Izzo V
Costa MA
Di Fiore R
Duro G
Bellavia D
Cascone E
Colombo P
Gioviale MC
Barbieri R
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Methodology

Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels

Vincenzo Izzo¹ , Maria A Costa¹ , Renata Di Fiore¹ , Giovanni Duro¹ , Daniele Bellavia² , Eleonora Cascone² , Paolo Colombo¹ , Maria C Gioviale³ and Rainer Barbieri²

¹Istituto di Biomedicina e Immunologia Molecolare, C.N.R., Via U. La Malfa, 153, 90153 Palermo, Italy

²Dipartimento di Biologia Cellulare e dello Sviluppo – Università di Palermo, V.le delle Scienze, 90128 Palermo, Italy

³Dipartimento GEN.UR.TO. – Università di Palermo, Italy

author email corresponding author email

Immunity & Ageing 2006, 3:7doi:10.1186/1742-4933-3-7


Published:	12 July 2006

Abstract

An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms.