Methodology
Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels
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* Corresponding author: Rainer Barbieri barner@unipa.it
1 Istituto di Biomedicina e Immunologia Molecolare, C.N.R., Via U. La Malfa, 153, 90153 Palermo, Italy
2 Dipartimento di Biologia Cellulare e dello Sviluppo – Università di Palermo, V.le delle Scienze, 90128 Palermo, Italy
3 Dipartimento GEN.UR.TO. – Università di Palermo, Italy
Immunity & Ageing 2006, 3:7 doi:10.1186/1742-4933-3-7
Published: 12 July 2006Abstract
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms.